电离辐射通过转化趋化因子-β-介导的上皮-间质转换来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

摘要 ：探讨电磁辐射是否可通过转化生长因子-β（TGF-β）-介导的角质层-间质转换 (EMT)来促进恶性肿瘤的侵袭移入。使用年均2Gy(60)Coγ线紫外线源自人类文明器官的6种恶性肿瘤，记事与EMT之外的变动，这仅限于分别利用孔径应用，抗原质印迹方法则，免疫荧光应用，划痕次测试和Transwell小室次测试来观察并扫描抗原组织形态，EMT上标，侵袭移入灵活性等。采用肽联免疫吸附法则扫描这些恶性肿瘤当中TGF-β抗原准确度，利用特别抑制作用剂SB431542来分析TGF-β信号通路在电磁辐射EMT当中的作用。经过年均为2Gy紫外线的恶性肿瘤当中依赖于间叶抗原的暗示，与假紫外线组相对于其角质层上标减少，间叶抗原上标降低，同时其侵袭移出灵活性提升，TGF-β抗原准确度也降低。必要性断定由A549电磁辐射其会的EMT可通过对TGF-β信号抑制作用发生再一。这些分析表明TGF-β介导的EMT在电磁辐射其会提升恶性肿瘤侵袭移出灵活性当中起着关键作用。

总编： lizexiu